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ETAD methods

Since its foundation, ETAD has accompanied its activity of monitoring ecological and toxicological issues on colorants with the development of corresponding methods for the determination of substances and properties of concern.

Thanks to the expertise of ETAD’s member companies, it has been possible to develop methods which suit the specific chemicophysical properties of dyes and organic pigments and allow a correct assessment of their impact on health and the environment.

Below is the full list of ETAD Methods. Kindly be informed that non-ETAD members can purchase access to these methods by contacting us directly via

For additional inquiries, please do not hesitate to reach out to us.

ETAD Toxicological Methods
No. 001*Determination of the acute oral toxicity (LD50).
No. 002*Examination for primary skin irritant action.
No. 003*Examination of irritant action on the eye.
No. 004Protocol for Salmonella / microsome test (Ames test).
No. 005, 1980Revised method for Salmonella / microsome test (Ames test).
No. 006Determination of acute inhalation toxicity of dusts, 1980.

*now superseded by OECD methods.

ETAD Analytical Methods (PCBs)
A 4003-214-3/1979Determination of polychlorobiphenyls (PCBs) in copper phthalocyanine, 1979.
A 4003-218-1/1986Determination of polychlorobiphenyls (PCBs) in copper phthalocyanine using reduction to biphenyl, 1986.

A 4003-219-9/1979


Determination of polychlorobiphenyls (PCBs) typically present in trace amounts in 3,3′-dichlorobenzidine-based pigments, 1979.
A 4003-222-8/1981Determination of decachlorobiphenyl in fully chlorinated phthalocyanines, 1981.
A 4003-223-7/1985Determination of monochlorobiphenyls in copper phthalocyanine, 1985.
A 4003-224-9/1986Determination of polychlorinated biphenyls (PCBs) by perchlorination, 1986.
A 4003-3/1989Guidelines for the determination of inadvertent polychlorinated biphenyls (PCBs) in colorants, 1989.
A 4003-226-12/1987Determination of the maximum possible level of polychlorobiphenyls (PCBs) and polychloroterphenyls (PCTs) in colorants by perchlorination, 1987.
No 229     NEW
Determination of total content of polychlorinated biphenyls in pigments by dissolution, cleanup and GC/MS, 2011.
M 1/8-1/1987Isolation of chlorinated biphenyls from colorants insoluble in water and in organic solvents via Soxhlet extraction, 1987.
M 1/9-1/1987Isolation of lipophilic impurities (e.g., PCBs) from colorants via liquid/liquid extraction, 1987.
M 1/10-1/1987Isolation and clean-up of polychlorinated biphenyls (PCBs) from colorants via column chromatography, 1987.
M 3/9-8/1990Determination of PCBs in colorants by combined GC-MS detection, 1990.
M 3/10-8/1990Experience with Sistovaris and Donges’ Method for determination of adventitious PCBs and PCTs in colorants. Report by Analytical Subcommittee, 1990.
ETAD Ecological Methods
No. 101A simple method of determining the biodegradability of products and contaminants in waste water.

Static method (R. Zahn and A. Wellens), 1975.

No. 102Continuous activated sludge test.

Modified OECD-confirmatory test for the determination of biological elimination. (Also known under the recent designation of OECD dynamic simulation test), 1975.

No. 103A screening test for the assessment of the possible inhibitory effect of a chemical substance on aerobic wastewater bacteria, 1979.
No. 104Method of evaluation of inhibition of microbial respiratory activity of the microorganisms of activated sludges and the microorganisms of rivers, 1979.
No. 105A screening test for assessing the primary anaerobic biodegradability of water-soluble dyestuffs, 1982.
ETAD Analytical Methods (general)
No. 201Method for the determination of α- and β-naphthylamine in C. I. Solvent Brown 1/2,1975.
No. 202Method for the determination of α- and β-naphthylamine in C. I. Acid Black 7. This method is suitable for water-soluble dyestuffs, 1976.
No. 203Determination of C. I. Disperse Blue 295 in mirror carp (Cyprinus carpio L.), 1976.
No. 204The accumulation of C. I. Acid Yellow 222 in the bodies of various types of fish, 1976.
No. 205Determination of disperse dyes in fish, 1976.
No. 206Determination of C. I. Basic Yellow 49 in fish, 1976.
No. 207Determination of the accumulation of C. I. Reactive Red 158 in the bodies of golden orfe, 1976.
No. 208Determination of the accumulation of C. I. Reactive Blue 121 in fish, 1976.
No. 209Determination of Direct Black ANBN in the presence of whole fish (goldfish and carp), 1976.
No. 210The determination of reactive dyes in dilute aqueous solution.

Range: 0.1 to 1 ppm, 1976.

No. 211Accumulation of dye in fish; methods of determination for chromium, nickel and cobalt complex dyestuffs, 1976.
No. 212Proposed method for the determination of unsulphonated primary aromatic amines in pigments and in solvent-soluble dyestuffs intended for use in food packaging materials, 1979.
No. 212 revisedIdentification and Quantification of Primary Aromatic Amines in Organic Pigments by HPLC. Edition May 3rd, 2016

The updated version allows a more precise quantification of residual PAAs.

No. 213Determination of reactive and acid dyes in fish (carp), 1979.
No. 215Determination of 4-aminobiphenyl in C. I. Solvent Black 5, 1979.
No. 216Determination of C. I. Acid Green 108 in fish flesh (carp) using high performance liquid chromatography, 1980.
No. 217Determination of 4-aminobiphenyl and benzidine in C. I. Solvent Black 5 (Nigrosines and Indulines), 1979.
No. 221Quantification of the limits of “no visible migration” of synthetic organic pigments under conditions of testing using the contact paper method,1980.
No. 225Determination of Cr(VI) traces in water-soluble Cr(III)-complex dyes by ion-chromatography, 1987.
Supplement to No. 225An analytical procedure to determine the level of extractable chromium in solvent soluble chromium(III) complex dyestuffs, 1987.
No. 227Monitoring of dyestuffs in drinking water, 1990.
No. 228Measurement of inspirable dyestuff dusts in the dyehouse atmosphere, 1991.