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ETAD methods

Since its foundation, ETAD has accompanied its activity of monitoring ecological and toxicological issues on colorants with the development of corresponding methods for the determination of substances and properties of concern.

Thanks to the expertise of ETAD’s member companies, it has been possible to develop methods which suit the specific chemicophysical properties of dyes and organic pigments and allow a correct assessment of their impact on health and the environment.

The complete list of ETAD Methods can be read below or downloaded as a pdf file.

For further information please contact us.

ETAD Toxicological Methods

No. 001*

Determination of the acute oral toxicity (LD50).

No. 002*

Examination for primary skin irritant action.

No. 003*

Examination of irritant action on the eye.

No. 004

Protocol for Salmonella / microsome test (Ames test).

No. 005, 1980

Revised method for Salmonella / microsome test (Ames test).

No. 006

Determination of acute inhalation toxicity of dusts, 1980.


*now superseded by OECD methods.


ETAD Analytical Methods (PCBs)

A 4003-214-3/1979

Determination of polychlorobiphenyls (PCBs) in copper phthalocyanine, 1979.

A 4003-218-1/1986

Determination of polychlorobiphenyls (PCBs) in copper phthalocyanine using reduction to biphenyl, 1986.

A 4003-219-9/1979


Determination of polychlorobiphenyls (PCBs) typically present in trace amounts in 3,3'-dichlorobenzidine-based pigments, 1979.

A 4003-222-8/1981

Determination of decachlorobiphenyl in fully chlorinated phthalocyanines, 1981.

A 4003-223-7/1985

Determination of monochlorobiphenyls in copper phthalocyanine, 1985.

A 4003-224-9/1986

Determination of polychlorinated biphenyls (PCBs) by perchlorination, 1986.

A 4003-3/1989

Guidelines for the determination of inadvertent polychlorinated biphenyls (PCBs) in colorants, 1989.

A 4003-226-12/1987

Determination of the maximum possible level of polychlorobiphenyls (PCBs) and polychloroterphenyls (PCTs) in colorants by perchlorination, 1987.

No 229     NEW

Determination of total content of polychlorinated biphenyls in pigments by dissolution, cleanup and GC/MS, 2011.

M 1/8-1/1987

Isolation of chlorinated biphenyls from colorants insoluble in water and in organic solvents via Soxhlet extraction, 1987.

M 1/9-1/1987

Isolation of lipophilic impurities (e.g., PCBs) from colorants via liquid/liquid extraction, 1987.

M 1/10-1/1987

Isolation and clean-up of polychlorinated biphenyls (PCBs) from colorants via column chromatography, 1987.

M 3/9-8/1990

Determination of PCBs in colorants by combined GC-MS detection, 1990.

M 3/10-8/1990

Experience with Sistovaris and Donges' Method for determination of adventitious PCBs and PCTs in colorants. Report by Analytical Subcommittee, 1990.


ETAD Ecological Methods

No. 101

A simple method of determining the biodegradability of products and contaminants in waste water.

Static method (R. Zahn and A. Wellens), 1975.

No. 102

Continuous activated sludge test.

Modified OECD-confirmatory test for the determination of biological elimination. (Also known under the recent designation of OECD dynamic simulation test), 1975.

No. 103

A screening test for the assessment of the possible inhibitory effect of a chemical substance on aerobic wastewater bacteria, 1979.

No. 104

Method of evaluation of inhibition of microbial respiratory activity of the microorganisms of activated sludges and the microorganisms of rivers, 1979.

No. 105

A screening test for assessing the primary anaerobic biodegradability of water-soluble dyestuffs, 1982.


ETAD Analytical Methods (general)

No. 201

Method for the determination of α- and β-naphthylamine in C. I. Solvent Brown 1/2,1975.

No. 202

Method for the determination of α- and β-naphthylamine in C. I. Acid Black 7. This method is suitable for water-soluble dyestuffs, 1976.

No. 203

Determination of C. I. Disperse Blue 295 in mirror carp (Cyprinus carpio L.), 1976.

No. 204

The accumulation of C. I. Acid Yellow 222 in the bodies of various types of fish, 1976.

No. 205

Determination of disperse dyes in fish, 1976.

No. 206

Determination of C. I. Basic Yellow 49 in fish, 1976.

No. 207

Determination of the accumulation of C. I. Reactive Red 158 in the bodies of golden orfe, 1976.

No. 208

Determination of the accumulation of C. I. Reactive Blue 121 in fish, 1976.

No. 209

Determination of Direct Black ANBN in the presence of whole fish (goldfish and carp), 1976.

No. 210

The determination of reactive dyes in dilute aqueous solution.

Range: 0.1 to 1 ppm, 1976.

No. 211

Accumulation of dye in fish; methods of determination for chromium, nickel and cobalt complex dyestuffs, 1976.

No. 212

Proposed method for the determination of unsulphonated primary aromatic amines in pigments and in solvent-soluble dyestuffs intended for use in food packaging materials, 1979.

No. 212 revised

Identification and Quantification of Primary Aromatic Amines in Organic Pigments by HPLC. Edition May 3rd, 2016

The updated version allows a more precise quantification of residual PAAs.

No. 213

Determination of reactive and acid dyes in fish (carp), 1979.

No. 215

Determination of 4-aminobiphenyl in C. I. Solvent Black 5, 1979.

No. 216

Determination of C. I. Acid Green 108 in fish flesh (carp) using high performance liquid chromatography, 1980.

No. 217

Determination of 4-aminobiphenyl and benzidine in C. I. Solvent Black 5 (Nigrosines and Indulines), 1979.

No. 221

Quantification of the limits of “no visible migration" of synthetic organic pigments under conditions of testing using the contact paper method,1980.

No. 225

Determination of Cr(VI) traces in water-soluble Cr(III)-complex dyes by ion-chromatography, 1987.

Supplement to No. 225

An analytical procedure to determine the level of extractable chromium in solvent soluble chromium(III) complex dyestuffs, 1987.

No. 227

Monitoring of dyestuffs in drinking water, 1990.

No. 228

Measurement of inspirable dyestuff dusts in the dyehouse atmosphere, 1991.